CNV System

Precision delivery of laser energy to the mouse and rat retina

Phoenix Research Labs’ Image-Guided Laser System produces precise, easy to deliver laser photocoagulation to generate choroidal neovascularization (CNV). Its user-friendly design enables technical staff to precisely and reliably control the location, size, and intensity of the laser photocoagulation required for CNV. Reliable creation of CNV has been validated in multiple studies.

CNV System Benefits:

  • Accurate control and documentation of delivery location
  • Precise control of spot size
  • Easy to use

Compact, precise laser delivery

Use the bright field image as a guide to place the aiming beam on target areas. Easily capture photographs or videos for publications or presentations to strengthen research documentation. Integration with the Phoenix Micron IV Retinal Imaging Microscope enables the recording of pre/post laser treatment spots as well as documentation of longitudinal studies.

Before using the Micron, our success rate at producing laser-induced choroidal neovascular lesions was ~40-50%; however, thanks to a successful implementation with the Micron, we now achieve a regular success rate of 99%.

Rafal Farjo, Ph.D.
Chief Operating Officer
Oklahoma City, OK  73104

Real-time Targeting for Photocoagulation

Laser targeting using image-guidance is much easier and more precise due to the magnification of the bright field image. Simply align the animal to the Micron, position and focus the aiming beam,adjust the laser settings and deliver the energy.

Combine our laser injector with Image-guided OCT for a new dimension in CNV studies

Phoenix Research Labs image-guided OCT2 provides unique insights into the process the of reliable CNV and assessing its progress. For immediate assessment from the bright field or angiogram, an OCT scan can be precisely located over of the center of the energy deposition to assess breaking of the Bruch’s membrane (Fig. 1). For more detail, a 3D representation (Fig 2) can be obtained. Success can be evaluated immediately and this is especially helpful when working with a large cohort of animals.
While the image-guided OCT from angiograms is quite useful, additional 3D information can be obtained and vertical scans (Fig 3) can be used to delineate the extent of neovascularization and layer location.

 Pre shot retina inspection

Pre shot retina inspection

 (Fig. 1) Post shot retinal inspection reveals the breaking of Bruch’s membrane.

(Fig. 1) Post shot retinal inspection reveals the breaking of Bruch’s membrane.

Laser injection on a Brown Norway rat

(Fig. 2) Post shot OCT immediately verifies breaking of Bruch’s membrane. Our InSight software details layers in this 3D representation.

CNV development is verified post laser using the OCT

(Fig. 3) Horizontal plane scanning vertically into the retina about 10 days post laser. Generation of CNV is clearly verified in the OCT 3D scan plan in the posterior retina.

Recent Phoenix Research Labs Webinar: Optimizing Image-Guided Laser-Induced Choroidal Neovascularization in Mice

Dr. Yan Gong and the team from the laboratory of Dr. Lois E. Smith, at Harvard Medical School, have completed and documented a study on laser-induced CNV using the Phoenix Research Labs CNV System, which was instrumental in achieving consistent and reproducible results. During this webinar, Dr. Gong reviews the new guidelines developed by the Harvard team in detail, from proper mouse selection, to the use of image-guided OCT to study the CNV process.

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